Synthetic and Artificial Chromosome Technologies: Methods and Protocols – Hardcover

This volume explores methodologies for the construction, assembly, delivery, and analysis of synthetic and artificial chromosomes in yeast, mammalian cells, plants, and other experimental systems. The chapters in this book are organized into five parts: Part I – Foundations and Construction of Synthetic and Artificial Chromosomes introduces methodologies for chromosome assembly and construction in yeast systems; Part II – BAC Technologies and Large DNA Engineering focuses on bacterial artificial chromosome technologies and large DNA engineering; Part III – Human Artificial Chromosomes and Mammalian Systems presents techniques for generating, delivering, and applying human artificial chromosomes and other megabase-scale DNA delivery platforms in mammalian cells and animal models; Part IV – Artificial Chromosome Function and Analysis describes approaches for investigating chromosome stability, centromere biology, kinetochore assembly, and chromosome function; and Part V – Advanced Applications of Synthetic and Artificial Chromosome Technologies highlights the expanding scope of chromosome engineering through methodologies involving viral genome engineering, plant episomal systems, and other emerging chromosome-scale engineering platforms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Synthetic and Artificial Chromosome Technologies: Methods and Protocols is a valuable reference for researchers, graduate students, and biotechnology scientists working in molecular genetics, chromosome biology, genome engineering, synthetic biology, and related disciplines. This book contributes to the continued advancement of synthetic and artificial chromosome technologies and supports future innovations in biotechnology, synthetic biology, and genome engineering.